![]() Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes. Gong W, Shen YP, Ma LG, Pan Y, Du YL, Wang DH, Yang JY, Hu LD, Liu XF, Dong CX, et al. Protein sequence names are shown on the left The key domains are highlighted, and the domain names are shown at the top of the sequence. The number above the sequence indicates the location of the amino acids in the BBX proteins. d Multiple sequence alignment of PeBBX protein sequences. Green boxes represent CCT domains, light purple boxes indicate BOX1 domains and yellow boxes indicate BOX2 domains. The length of each protein sequence is represented by the grey bars, and colored boxes represent conserved domains. Conserved domain predictions for the 27 PeBBX proteins. The scale bar at the bottom indicates the protein lengths, and sequence logos for each conserved motif are shown on the right. Distribution of conserved motifs in PeBBX proteins. Yellow boxes represent exons (CDS), black lines represent introns and green boxes represent the 5’ and 3’ untranslated regions. Intron-exon structure of the PeBBX genes. Gene structure, motifs, conserved structural domains, compositions and multiple sequence alignment analysis of the PeBBX gene family. In conclusion, we analyzed the PeBBX genes at multiple different levels, which will contribute to further studies of the BBX family and provide valuable information for the functional validation of this family.Ĭharacterization Expression analysis Gene family Moso bamboo. We further explored the downstream target genes of PeBBXs, and GO/KEGG enrichment analysis predicted multiple functions of BBX target genes, including those encoding enzymes involved in plant photosynthesis, pyruvate metabolism and glycolysis/gluconeogenesis. Short time-series expression miner (STEM) analysis revealed that the PeBBX genes had spatiotemporal-specific expression patterns and were likely involved in the growth and development of bamboo shoots. Light-responsive and stress-related cis-elements were identified by analyzing cis-elements in the promoters of all PeBBX genes. Additionally, phylogenetic and homology analyses indicated that PeBBX genes were divided into three clusters, with whole-genome duplication (WGD) events having facilitated the expansion of this gene family. In the present study, we identified 27 BBX genes in the genome of Moso bamboo, and analysis of their conserved motifs and multiple sequence alignments revealed that they all shared highly similar structures. Although BBX genes have been characterized in many model organisms, genome-wide identification of the BBX family genes have not yet been reported in Moso bamboo (Phyllostachys edulis), and the biological functions of this family remain unknown. The BBX (B-box) family are zinc finger protein (ZFP) transcription factors that play an essential role in plant growth, development and response to abiotic stresses. ![]()
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